Journal: eLife
Article Title: Intraocular dendritic cells characterize HLA-B27-associated acute anterior uveitis
doi: 10.7554/eLife.67396
Figure Lengend Snippet: ( A ) Leukocytes in side scatter (SSC)/forward scatter (FSC) scatter were identified, doublets were excluded, and leukocytes were gated on CD3-CD11b+ myeloid cells, CD3+ CD11b- T cells, and CD3-CD11b- cells. Myeloid cells were further classified into CD11c+ HLA-DR- granulocytes and CD11c+ HLA-DR+ monocytes/macrophages/dendritic cells (DCs). T cells were further subdivided into CD4+- and CD8+-expressing cells. CD3-CD11b- cells were analyzed according to their frequency of CD56+ natural killer (NK) cells. Representative analysis is shown. ( B ) Correlation plot (Spearman’s correlation coefficient) between flow proportions (columns) and single-cell RNA-sequencing (scRNA-seq) (rows) proportions is shown as a heatmap, with high correlation coefficients shown in red.
Article Snippet: Commercial assay or kit , NextSeq 500/550 High Output Kit v2.5 (150 cycles) , Illumina , Cat# 20024907 , RNA-seq analysis.
Techniques: Expressing, RNA Sequencing Assay